Journal: International Journal of Molecular Sciences
Article Title: Mouse Model of Parkinson’s Disease with Bilateral Dorsal Striatum Lesion with 6-Hydroxydopamine Exhibits Cognitive Apathy-like Behavior
doi: 10.3390/ijms25147993
Figure Lengend Snippet: Three-chamber test. ( A ) Results of session 1, sociability test. ( B ) Results of session 2, social novelty preference. In small round wire cages in the left and right of three chambers, a 25 mm sponge cube (novel object) or a 14-week-old male C57BL/6J mouse with no prior contact with the test mouse was enclosed, respectively (session 1). The test mice were placed to the middle chamber and allowed to explore it for 10 min. To determine the interaction time, the total duration that the head of the test mouse was within the zone that was set at 30 mm around each of the cages was automatically counted using animal behavior analysis software. The interaction time for the novel object significantly decreased in the 6-OHDA group compared with the sham group, but there was no significant difference for the social object. After the session, the 14-week-old male C57BL/6J mouse was again placed in the holding cage (familiar), and a second unfamiliar mouse (stranger) was enclosed in the other wire cage on the opposite side (session 2). The rest of the procedure was conducted in the same way as mentioned above for session 1. The interaction time for either the familiar or stranger mouse did not significantly differ between the two groups. The data are expressed as the mean ± SEM with data point overlap. * p < 0.05.
Article Snippet: The three-chamber test was conducted using a sociability apparatus (SC-03M, Muromachi Kikai) to assess sociability and social novelty preference [ , ].
Techniques: Software